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kat2a primary cell based screening assays cell culture ht1080 human fibrosarcoma cell line ht1080  (ATCC)


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    ATCC kat2a primary cell based screening assays cell culture ht1080 human fibrosarcoma cell line ht1080
    Kat2a Primary Cell Based Screening Assays Cell Culture Ht1080 Human Fibrosarcoma Cell Line Ht1080, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 4052 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+fibrosarcoma+cell+line/HT-1080/us12655105-1783-0-16
    Average 98 stars, based on 4052 article reviews
    kat2a primary cell based screening assays cell culture ht1080 human fibrosarcoma cell line ht1080 - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Transformation Assay:

    Article Title: Methods and compositions for delivering mRNA coded antibodies
    Article Snippet: .. Non-limiting examples of mammalian cells include BALB/c mouse myeloma line (NSO/1, ECACC No: 85110503); human retinoblasts (PER.C6, CruCell, Leiden, The Netherlands); monkey kidney CV1 line transformed by SV40 (COS-7, ATCC CRL 1651); human embryonic kidney line (HEK293 or 293 cells subcloned for growth in suspension culture, Graham et al., J. Gen Virol., 36:59, 1977); human fibrosarcoma cell line (e.g., HT1080); baby hamster kidney cells (BHK21, ATCC CCL 10); Chinese hamster ovary cells +/−DHFR (CHO, Urlaub and Chasin, Proc. ..

    Article Title: Methods and compositions for treatment of hunter syndrome comprising iduronate-2-sulfatase
    Article Snippet: .. Non-limiting examples of mammalian cells that may be used in accordance with the present invention include BALB/c mouse myeloma line (NSO/1, ECACC No: 85110503); human retinoblasts (PER.C6, CruCell, Leiden, The Netherlands); monkey kidney CV1 line transformed by SV40 (COS-7, ATCC CRL 1651); human embryonic kidney line (HEK293 or 293 cells subcloned for growth in suspension culture, Graham et al., J. Gen Virol., 36:59,1977); human fibrosarcoma cell line (e.g., HT1080); baby hamster kidney cells (BHK21, ATCC CCL 10); Chinese hamster ovary cells+/−DHFR (CHO, Urlaub and Chasin, Proc. ..

    Suspension:

    Article Title: Methods and compositions for delivering mRNA coded antibodies
    Article Snippet: .. Non-limiting examples of mammalian cells include BALB/c mouse myeloma line (NSO/1, ECACC No: 85110503); human retinoblasts (PER.C6, CruCell, Leiden, The Netherlands); monkey kidney CV1 line transformed by SV40 (COS-7, ATCC CRL 1651); human embryonic kidney line (HEK293 or 293 cells subcloned for growth in suspension culture, Graham et al., J. Gen Virol., 36:59, 1977); human fibrosarcoma cell line (e.g., HT1080); baby hamster kidney cells (BHK21, ATCC CCL 10); Chinese hamster ovary cells +/−DHFR (CHO, Urlaub and Chasin, Proc. ..

    Article Title: Methods and compositions for treatment of hunter syndrome comprising iduronate-2-sulfatase
    Article Snippet: .. Non-limiting examples of mammalian cells that may be used in accordance with the present invention include BALB/c mouse myeloma line (NSO/1, ECACC No: 85110503); human retinoblasts (PER.C6, CruCell, Leiden, The Netherlands); monkey kidney CV1 line transformed by SV40 (COS-7, ATCC CRL 1651); human embryonic kidney line (HEK293 or 293 cells subcloned for growth in suspension culture, Graham et al., J. Gen Virol., 36:59,1977); human fibrosarcoma cell line (e.g., HT1080); baby hamster kidney cells (BHK21, ATCC CCL 10); Chinese hamster ovary cells+/−DHFR (CHO, Urlaub and Chasin, Proc. ..

    Derivative Assay:

    Article Title: Calcium Spirulan from <i>Arthrospira platensis</i> (Spirulina) Prevents Herpes simplex Virus Type I Cell Entry <i>in vitro</i>
    Article Snippet: .. Taken together, our results support the use of CaSP in the topical prophylaxis and treatment of recurrent HSV-1 infections.42 Abbreviations ACV acyclovir ANOVA analysis of variance B16-BL6 murine melanoma cell line derived from the B16-F10 line BSA bovine serum albumin C18 column octadecylsilyl (ODS) reversed-phase HPLC column CaSP calcium spirulan CPE cytopathic effect Colon 26-M3.1 cells a highly metastatic subline of the murine Colon 26 (C26) carcinoma DAB 3,3′-Diaminobenzidine DAD Diode Array Detector DMEM Dulbecco’s Modified Eagle Medium EC50 half-maximal effective concentrations EDTA ethylenediaminetetraacetic acid FBS fetal bovine serum FD freeze drying HaCaT cells Human adult low-Calcium-activated, Temperature-sensitive keratinocyte cell line HeLa cells Henrietta Lacks cervical cancer cell line HRP Horseradish peroxidase HIV human immunodeficiency virus HPL human platelet lysate HSV-1 Herpes simplex virus type 1 HT-1080 cells a human fibrosarcoma cell line (ATCC® CCL-121TM) HPLC High-Performance Liquid Chromatography HPLC-RI HPLC-Refractive Index detector HRP Rabbit anti-goat IgG H&L IHC Immunohistochemical colorimetric staining m/z mass-to-charge ratio MS mass spectrometry NCPV National Collection of Pathogenic Viruses Nrf-2 Nuclear factor erythroid 2-related factor 2 Nucleogel Sugar 810 H column Sulfonated spherical polystyrene resin, crosslinked with divinylbenzene (PS/DVB), H+ form p.A. per Analysis PBS phosphate-buffered saline RT room temperature SDS sodium dodecyl sulfate TCID50 median tissue culture infectious Dose t PA tissue-type plasminogen activator Tris tris-(hydroxymethyl) aminomethane Triton X-100 polyethylene glycol p-(1,1,3,3-tetramethylbutyl)-phenyl ether UPLC ultra-high-performance liquid chromatography UV/VIS detector Ultraviolet/Visible detector w/v weight/volume XXT 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H- tetrazolium-5-carboxanilide. ..

    High Performance Liquid Chromatography:

    Article Title: Calcium Spirulan from <i>Arthrospira platensis</i> (Spirulina) Prevents Herpes simplex Virus Type I Cell Entry <i>in vitro</i>
    Article Snippet: .. Taken together, our results support the use of CaSP in the topical prophylaxis and treatment of recurrent HSV-1 infections.42 Abbreviations ACV acyclovir ANOVA analysis of variance B16-BL6 murine melanoma cell line derived from the B16-F10 line BSA bovine serum albumin C18 column octadecylsilyl (ODS) reversed-phase HPLC column CaSP calcium spirulan CPE cytopathic effect Colon 26-M3.1 cells a highly metastatic subline of the murine Colon 26 (C26) carcinoma DAB 3,3′-Diaminobenzidine DAD Diode Array Detector DMEM Dulbecco’s Modified Eagle Medium EC50 half-maximal effective concentrations EDTA ethylenediaminetetraacetic acid FBS fetal bovine serum FD freeze drying HaCaT cells Human adult low-Calcium-activated, Temperature-sensitive keratinocyte cell line HeLa cells Henrietta Lacks cervical cancer cell line HRP Horseradish peroxidase HIV human immunodeficiency virus HPL human platelet lysate HSV-1 Herpes simplex virus type 1 HT-1080 cells a human fibrosarcoma cell line (ATCC® CCL-121TM) HPLC High-Performance Liquid Chromatography HPLC-RI HPLC-Refractive Index detector HRP Rabbit anti-goat IgG H&L IHC Immunohistochemical colorimetric staining m/z mass-to-charge ratio MS mass spectrometry NCPV National Collection of Pathogenic Viruses Nrf-2 Nuclear factor erythroid 2-related factor 2 Nucleogel Sugar 810 H column Sulfonated spherical polystyrene resin, crosslinked with divinylbenzene (PS/DVB), H+ form p.A. per Analysis PBS phosphate-buffered saline RT room temperature SDS sodium dodecyl sulfate TCID50 median tissue culture infectious Dose t PA tissue-type plasminogen activator Tris tris-(hydroxymethyl) aminomethane Triton X-100 polyethylene glycol p-(1,1,3,3-tetramethylbutyl)-phenyl ether UPLC ultra-high-performance liquid chromatography UV/VIS detector Ultraviolet/Visible detector w/v weight/volume XXT 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H- tetrazolium-5-carboxanilide. ..

    Modification:

    Article Title: Calcium Spirulan from <i>Arthrospira platensis</i> (Spirulina) Prevents Herpes simplex Virus Type I Cell Entry <i>in vitro</i>
    Article Snippet: .. Taken together, our results support the use of CaSP in the topical prophylaxis and treatment of recurrent HSV-1 infections.42 Abbreviations ACV acyclovir ANOVA analysis of variance B16-BL6 murine melanoma cell line derived from the B16-F10 line BSA bovine serum albumin C18 column octadecylsilyl (ODS) reversed-phase HPLC column CaSP calcium spirulan CPE cytopathic effect Colon 26-M3.1 cells a highly metastatic subline of the murine Colon 26 (C26) carcinoma DAB 3,3′-Diaminobenzidine DAD Diode Array Detector DMEM Dulbecco’s Modified Eagle Medium EC50 half-maximal effective concentrations EDTA ethylenediaminetetraacetic acid FBS fetal bovine serum FD freeze drying HaCaT cells Human adult low-Calcium-activated, Temperature-sensitive keratinocyte cell line HeLa cells Henrietta Lacks cervical cancer cell line HRP Horseradish peroxidase HIV human immunodeficiency virus HPL human platelet lysate HSV-1 Herpes simplex virus type 1 HT-1080 cells a human fibrosarcoma cell line (ATCC® CCL-121TM) HPLC High-Performance Liquid Chromatography HPLC-RI HPLC-Refractive Index detector HRP Rabbit anti-goat IgG H&L IHC Immunohistochemical colorimetric staining m/z mass-to-charge ratio MS mass spectrometry NCPV National Collection of Pathogenic Viruses Nrf-2 Nuclear factor erythroid 2-related factor 2 Nucleogel Sugar 810 H column Sulfonated spherical polystyrene resin, crosslinked with divinylbenzene (PS/DVB), H+ form p.A. per Analysis PBS phosphate-buffered saline RT room temperature SDS sodium dodecyl sulfate TCID50 median tissue culture infectious Dose t PA tissue-type plasminogen activator Tris tris-(hydroxymethyl) aminomethane Triton X-100 polyethylene glycol p-(1,1,3,3-tetramethylbutyl)-phenyl ether UPLC ultra-high-performance liquid chromatography UV/VIS detector Ultraviolet/Visible detector w/v weight/volume XXT 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H- tetrazolium-5-carboxanilide. ..

    Article Title: IL12-engineered human PSMA-CAR T cells for the treatment of advanced prostate cancer
    Article Snippet: .. CWR22Rv1 (CRL-2505) (a kind gift from Dr. Robert Reiter, UCLA) and LNCaP were cultured in complete RPMI The human fibrosarcoma cell line, HT1080 (ATCC CCL-12), and the human embryonic kidney cell line, 293T (ATCC CRL-3216), were cultured in Dulbecco’s Modified Eagles Medium (DMEM, Life Technologies) containing 10% FBS, 1X AA, 25 mM HEPES (Irvine Scientific), and 2 mM L-Glutamine (CorningTM, complete DMEM). .. The human prostate cancer-derived xenograft LAPC-9 (a kind gift from Dr. Robert Reiter, UCLA) was serially passaged in male NOD.Cg-Prkdcscid IL2rgtm1Wjl/SzJ (NSG) mice, and single-cell suspensions were prepared using the Miltenyi Biotec human tumor dissociation kit (cat: 130-095-929) per manufacturer’s protocol.

    Article Title: Chimeric antigen receptors targeted to PSCA
    Article Snippet: Cell Lines: (Human metastatic prostate cancer cell lines DU145 (ATCC HTB-81) and PC-3 (ATCC CRL-1435) were cultured in RPMI-1640 (Lonza) containing 10% fetal bovine serum (FBS, Hyclone), and 1× antibiotic-antimycotic (Gibco) containing 100 U/mL penicillin, 100 μg/mL streptomycin, and 0.25 ug/mL fungizone (complete RPMI). .. The human fibrosarcoma cell line, HT1080 (ATCC CCL-121), and the human embryonic kidney cell line, 293T (ATCC CRL-3216), were cultured in Dulbecco's Modified Eagles Medium (DMEM, Life Technologies) containing 10% FBS, 1× antibiotic-antimycotic, 25 mM HEPES (Irvine Scientific), and 2 mM L-Glutamine (Fisher Scientific) (complete DMEM). .. The human prostate cancer xenograft LAPC-9 (a kind gift from Dr. Robert Reiter, UCLA) was cultured in Iscove's Modified Dulbecco's Medium (IMDM, Irvine Scientific) containing 20% FBS and 1× antibiotic-antimycotic (complete IMDM).

    Virus:

    Article Title: Calcium Spirulan from <i>Arthrospira platensis</i> (Spirulina) Prevents Herpes simplex Virus Type I Cell Entry <i>in vitro</i>
    Article Snippet: .. Taken together, our results support the use of CaSP in the topical prophylaxis and treatment of recurrent HSV-1 infections.42 Abbreviations ACV acyclovir ANOVA analysis of variance B16-BL6 murine melanoma cell line derived from the B16-F10 line BSA bovine serum albumin C18 column octadecylsilyl (ODS) reversed-phase HPLC column CaSP calcium spirulan CPE cytopathic effect Colon 26-M3.1 cells a highly metastatic subline of the murine Colon 26 (C26) carcinoma DAB 3,3′-Diaminobenzidine DAD Diode Array Detector DMEM Dulbecco’s Modified Eagle Medium EC50 half-maximal effective concentrations EDTA ethylenediaminetetraacetic acid FBS fetal bovine serum FD freeze drying HaCaT cells Human adult low-Calcium-activated, Temperature-sensitive keratinocyte cell line HeLa cells Henrietta Lacks cervical cancer cell line HRP Horseradish peroxidase HIV human immunodeficiency virus HPL human platelet lysate HSV-1 Herpes simplex virus type 1 HT-1080 cells a human fibrosarcoma cell line (ATCC® CCL-121TM) HPLC High-Performance Liquid Chromatography HPLC-RI HPLC-Refractive Index detector HRP Rabbit anti-goat IgG H&L IHC Immunohistochemical colorimetric staining m/z mass-to-charge ratio MS mass spectrometry NCPV National Collection of Pathogenic Viruses Nrf-2 Nuclear factor erythroid 2-related factor 2 Nucleogel Sugar 810 H column Sulfonated spherical polystyrene resin, crosslinked with divinylbenzene (PS/DVB), H+ form p.A. per Analysis PBS phosphate-buffered saline RT room temperature SDS sodium dodecyl sulfate TCID50 median tissue culture infectious Dose t PA tissue-type plasminogen activator Tris tris-(hydroxymethyl) aminomethane Triton X-100 polyethylene glycol p-(1,1,3,3-tetramethylbutyl)-phenyl ether UPLC ultra-high-performance liquid chromatography UV/VIS detector Ultraviolet/Visible detector w/v weight/volume XXT 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H- tetrazolium-5-carboxanilide. ..

    Immunohistochemistry:

    Article Title: Calcium Spirulan from <i>Arthrospira platensis</i> (Spirulina) Prevents Herpes simplex Virus Type I Cell Entry <i>in vitro</i>
    Article Snippet: .. Taken together, our results support the use of CaSP in the topical prophylaxis and treatment of recurrent HSV-1 infections.42 Abbreviations ACV acyclovir ANOVA analysis of variance B16-BL6 murine melanoma cell line derived from the B16-F10 line BSA bovine serum albumin C18 column octadecylsilyl (ODS) reversed-phase HPLC column CaSP calcium spirulan CPE cytopathic effect Colon 26-M3.1 cells a highly metastatic subline of the murine Colon 26 (C26) carcinoma DAB 3,3′-Diaminobenzidine DAD Diode Array Detector DMEM Dulbecco’s Modified Eagle Medium EC50 half-maximal effective concentrations EDTA ethylenediaminetetraacetic acid FBS fetal bovine serum FD freeze drying HaCaT cells Human adult low-Calcium-activated, Temperature-sensitive keratinocyte cell line HeLa cells Henrietta Lacks cervical cancer cell line HRP Horseradish peroxidase HIV human immunodeficiency virus HPL human platelet lysate HSV-1 Herpes simplex virus type 1 HT-1080 cells a human fibrosarcoma cell line (ATCC® CCL-121TM) HPLC High-Performance Liquid Chromatography HPLC-RI HPLC-Refractive Index detector HRP Rabbit anti-goat IgG H&L IHC Immunohistochemical colorimetric staining m/z mass-to-charge ratio MS mass spectrometry NCPV National Collection of Pathogenic Viruses Nrf-2 Nuclear factor erythroid 2-related factor 2 Nucleogel Sugar 810 H column Sulfonated spherical polystyrene resin, crosslinked with divinylbenzene (PS/DVB), H+ form p.A. per Analysis PBS phosphate-buffered saline RT room temperature SDS sodium dodecyl sulfate TCID50 median tissue culture infectious Dose t PA tissue-type plasminogen activator Tris tris-(hydroxymethyl) aminomethane Triton X-100 polyethylene glycol p-(1,1,3,3-tetramethylbutyl)-phenyl ether UPLC ultra-high-performance liquid chromatography UV/VIS detector Ultraviolet/Visible detector w/v weight/volume XXT 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H- tetrazolium-5-carboxanilide. ..

    Immunohistochemical staining:

    Article Title: Calcium Spirulan from <i>Arthrospira platensis</i> (Spirulina) Prevents Herpes simplex Virus Type I Cell Entry <i>in vitro</i>
    Article Snippet: .. Taken together, our results support the use of CaSP in the topical prophylaxis and treatment of recurrent HSV-1 infections.42 Abbreviations ACV acyclovir ANOVA analysis of variance B16-BL6 murine melanoma cell line derived from the B16-F10 line BSA bovine serum albumin C18 column octadecylsilyl (ODS) reversed-phase HPLC column CaSP calcium spirulan CPE cytopathic effect Colon 26-M3.1 cells a highly metastatic subline of the murine Colon 26 (C26) carcinoma DAB 3,3′-Diaminobenzidine DAD Diode Array Detector DMEM Dulbecco’s Modified Eagle Medium EC50 half-maximal effective concentrations EDTA ethylenediaminetetraacetic acid FBS fetal bovine serum FD freeze drying HaCaT cells Human adult low-Calcium-activated, Temperature-sensitive keratinocyte cell line HeLa cells Henrietta Lacks cervical cancer cell line HRP Horseradish peroxidase HIV human immunodeficiency virus HPL human platelet lysate HSV-1 Herpes simplex virus type 1 HT-1080 cells a human fibrosarcoma cell line (ATCC® CCL-121TM) HPLC High-Performance Liquid Chromatography HPLC-RI HPLC-Refractive Index detector HRP Rabbit anti-goat IgG H&L IHC Immunohistochemical colorimetric staining m/z mass-to-charge ratio MS mass spectrometry NCPV National Collection of Pathogenic Viruses Nrf-2 Nuclear factor erythroid 2-related factor 2 Nucleogel Sugar 810 H column Sulfonated spherical polystyrene resin, crosslinked with divinylbenzene (PS/DVB), H+ form p.A. per Analysis PBS phosphate-buffered saline RT room temperature SDS sodium dodecyl sulfate TCID50 median tissue culture infectious Dose t PA tissue-type plasminogen activator Tris tris-(hydroxymethyl) aminomethane Triton X-100 polyethylene glycol p-(1,1,3,3-tetramethylbutyl)-phenyl ether UPLC ultra-high-performance liquid chromatography UV/VIS detector Ultraviolet/Visible detector w/v weight/volume XXT 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H- tetrazolium-5-carboxanilide. ..

    Staining:

    Article Title: Calcium Spirulan from <i>Arthrospira platensis</i> (Spirulina) Prevents Herpes simplex Virus Type I Cell Entry <i>in vitro</i>
    Article Snippet: .. Taken together, our results support the use of CaSP in the topical prophylaxis and treatment of recurrent HSV-1 infections.42 Abbreviations ACV acyclovir ANOVA analysis of variance B16-BL6 murine melanoma cell line derived from the B16-F10 line BSA bovine serum albumin C18 column octadecylsilyl (ODS) reversed-phase HPLC column CaSP calcium spirulan CPE cytopathic effect Colon 26-M3.1 cells a highly metastatic subline of the murine Colon 26 (C26) carcinoma DAB 3,3′-Diaminobenzidine DAD Diode Array Detector DMEM Dulbecco’s Modified Eagle Medium EC50 half-maximal effective concentrations EDTA ethylenediaminetetraacetic acid FBS fetal bovine serum FD freeze drying HaCaT cells Human adult low-Calcium-activated, Temperature-sensitive keratinocyte cell line HeLa cells Henrietta Lacks cervical cancer cell line HRP Horseradish peroxidase HIV human immunodeficiency virus HPL human platelet lysate HSV-1 Herpes simplex virus type 1 HT-1080 cells a human fibrosarcoma cell line (ATCC® CCL-121TM) HPLC High-Performance Liquid Chromatography HPLC-RI HPLC-Refractive Index detector HRP Rabbit anti-goat IgG H&L IHC Immunohistochemical colorimetric staining m/z mass-to-charge ratio MS mass spectrometry NCPV National Collection of Pathogenic Viruses Nrf-2 Nuclear factor erythroid 2-related factor 2 Nucleogel Sugar 810 H column Sulfonated spherical polystyrene resin, crosslinked with divinylbenzene (PS/DVB), H+ form p.A. per Analysis PBS phosphate-buffered saline RT room temperature SDS sodium dodecyl sulfate TCID50 median tissue culture infectious Dose t PA tissue-type plasminogen activator Tris tris-(hydroxymethyl) aminomethane Triton X-100 polyethylene glycol p-(1,1,3,3-tetramethylbutyl)-phenyl ether UPLC ultra-high-performance liquid chromatography UV/VIS detector Ultraviolet/Visible detector w/v weight/volume XXT 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H- tetrazolium-5-carboxanilide. ..

    Mass Spectrometry:

    Article Title: Calcium Spirulan from <i>Arthrospira platensis</i> (Spirulina) Prevents Herpes simplex Virus Type I Cell Entry <i>in vitro</i>
    Article Snippet: .. Taken together, our results support the use of CaSP in the topical prophylaxis and treatment of recurrent HSV-1 infections.42 Abbreviations ACV acyclovir ANOVA analysis of variance B16-BL6 murine melanoma cell line derived from the B16-F10 line BSA bovine serum albumin C18 column octadecylsilyl (ODS) reversed-phase HPLC column CaSP calcium spirulan CPE cytopathic effect Colon 26-M3.1 cells a highly metastatic subline of the murine Colon 26 (C26) carcinoma DAB 3,3′-Diaminobenzidine DAD Diode Array Detector DMEM Dulbecco’s Modified Eagle Medium EC50 half-maximal effective concentrations EDTA ethylenediaminetetraacetic acid FBS fetal bovine serum FD freeze drying HaCaT cells Human adult low-Calcium-activated, Temperature-sensitive keratinocyte cell line HeLa cells Henrietta Lacks cervical cancer cell line HRP Horseradish peroxidase HIV human immunodeficiency virus HPL human platelet lysate HSV-1 Herpes simplex virus type 1 HT-1080 cells a human fibrosarcoma cell line (ATCC® CCL-121TM) HPLC High-Performance Liquid Chromatography HPLC-RI HPLC-Refractive Index detector HRP Rabbit anti-goat IgG H&L IHC Immunohistochemical colorimetric staining m/z mass-to-charge ratio MS mass spectrometry NCPV National Collection of Pathogenic Viruses Nrf-2 Nuclear factor erythroid 2-related factor 2 Nucleogel Sugar 810 H column Sulfonated spherical polystyrene resin, crosslinked with divinylbenzene (PS/DVB), H+ form p.A. per Analysis PBS phosphate-buffered saline RT room temperature SDS sodium dodecyl sulfate TCID50 median tissue culture infectious Dose t PA tissue-type plasminogen activator Tris tris-(hydroxymethyl) aminomethane Triton X-100 polyethylene glycol p-(1,1,3,3-tetramethylbutyl)-phenyl ether UPLC ultra-high-performance liquid chromatography UV/VIS detector Ultraviolet/Visible detector w/v weight/volume XXT 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H- tetrazolium-5-carboxanilide. ..

    Saline:

    Article Title: Calcium Spirulan from <i>Arthrospira platensis</i> (Spirulina) Prevents Herpes simplex Virus Type I Cell Entry <i>in vitro</i>
    Article Snippet: .. Taken together, our results support the use of CaSP in the topical prophylaxis and treatment of recurrent HSV-1 infections.42 Abbreviations ACV acyclovir ANOVA analysis of variance B16-BL6 murine melanoma cell line derived from the B16-F10 line BSA bovine serum albumin C18 column octadecylsilyl (ODS) reversed-phase HPLC column CaSP calcium spirulan CPE cytopathic effect Colon 26-M3.1 cells a highly metastatic subline of the murine Colon 26 (C26) carcinoma DAB 3,3′-Diaminobenzidine DAD Diode Array Detector DMEM Dulbecco’s Modified Eagle Medium EC50 half-maximal effective concentrations EDTA ethylenediaminetetraacetic acid FBS fetal bovine serum FD freeze drying HaCaT cells Human adult low-Calcium-activated, Temperature-sensitive keratinocyte cell line HeLa cells Henrietta Lacks cervical cancer cell line HRP Horseradish peroxidase HIV human immunodeficiency virus HPL human platelet lysate HSV-1 Herpes simplex virus type 1 HT-1080 cells a human fibrosarcoma cell line (ATCC® CCL-121TM) HPLC High-Performance Liquid Chromatography HPLC-RI HPLC-Refractive Index detector HRP Rabbit anti-goat IgG H&L IHC Immunohistochemical colorimetric staining m/z mass-to-charge ratio MS mass spectrometry NCPV National Collection of Pathogenic Viruses Nrf-2 Nuclear factor erythroid 2-related factor 2 Nucleogel Sugar 810 H column Sulfonated spherical polystyrene resin, crosslinked with divinylbenzene (PS/DVB), H+ form p.A. per Analysis PBS phosphate-buffered saline RT room temperature SDS sodium dodecyl sulfate TCID50 median tissue culture infectious Dose t PA tissue-type plasminogen activator Tris tris-(hydroxymethyl) aminomethane Triton X-100 polyethylene glycol p-(1,1,3,3-tetramethylbutyl)-phenyl ether UPLC ultra-high-performance liquid chromatography UV/VIS detector Ultraviolet/Visible detector w/v weight/volume XXT 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H- tetrazolium-5-carboxanilide. ..

    Liquid Chromatography:

    Article Title: Calcium Spirulan from <i>Arthrospira platensis</i> (Spirulina) Prevents Herpes simplex Virus Type I Cell Entry <i>in vitro</i>
    Article Snippet: .. Taken together, our results support the use of CaSP in the topical prophylaxis and treatment of recurrent HSV-1 infections.42 Abbreviations ACV acyclovir ANOVA analysis of variance B16-BL6 murine melanoma cell line derived from the B16-F10 line BSA bovine serum albumin C18 column octadecylsilyl (ODS) reversed-phase HPLC column CaSP calcium spirulan CPE cytopathic effect Colon 26-M3.1 cells a highly metastatic subline of the murine Colon 26 (C26) carcinoma DAB 3,3′-Diaminobenzidine DAD Diode Array Detector DMEM Dulbecco’s Modified Eagle Medium EC50 half-maximal effective concentrations EDTA ethylenediaminetetraacetic acid FBS fetal bovine serum FD freeze drying HaCaT cells Human adult low-Calcium-activated, Temperature-sensitive keratinocyte cell line HeLa cells Henrietta Lacks cervical cancer cell line HRP Horseradish peroxidase HIV human immunodeficiency virus HPL human platelet lysate HSV-1 Herpes simplex virus type 1 HT-1080 cells a human fibrosarcoma cell line (ATCC® CCL-121TM) HPLC High-Performance Liquid Chromatography HPLC-RI HPLC-Refractive Index detector HRP Rabbit anti-goat IgG H&L IHC Immunohistochemical colorimetric staining m/z mass-to-charge ratio MS mass spectrometry NCPV National Collection of Pathogenic Viruses Nrf-2 Nuclear factor erythroid 2-related factor 2 Nucleogel Sugar 810 H column Sulfonated spherical polystyrene resin, crosslinked with divinylbenzene (PS/DVB), H+ form p.A. per Analysis PBS phosphate-buffered saline RT room temperature SDS sodium dodecyl sulfate TCID50 median tissue culture infectious Dose t PA tissue-type plasminogen activator Tris tris-(hydroxymethyl) aminomethane Triton X-100 polyethylene glycol p-(1,1,3,3-tetramethylbutyl)-phenyl ether UPLC ultra-high-performance liquid chromatography UV/VIS detector Ultraviolet/Visible detector w/v weight/volume XXT 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H- tetrazolium-5-carboxanilide. ..

    Cell Culture:

    Article Title: IL12-engineered human PSMA-CAR T cells for the treatment of advanced prostate cancer
    Article Snippet: .. CWR22Rv1 (CRL-2505) (a kind gift from Dr. Robert Reiter, UCLA) and LNCaP were cultured in complete RPMI The human fibrosarcoma cell line, HT1080 (ATCC CCL-12), and the human embryonic kidney cell line, 293T (ATCC CRL-3216), were cultured in Dulbecco’s Modified Eagles Medium (DMEM, Life Technologies) containing 10% FBS, 1X AA, 25 mM HEPES (Irvine Scientific), and 2 mM L-Glutamine (CorningTM, complete DMEM). .. The human prostate cancer-derived xenograft LAPC-9 (a kind gift from Dr. Robert Reiter, UCLA) was serially passaged in male NOD.Cg-Prkdcscid IL2rgtm1Wjl/SzJ (NSG) mice, and single-cell suspensions were prepared using the Miltenyi Biotec human tumor dissociation kit (cat: 130-095-929) per manufacturer’s protocol.

    Article Title: Chimeric antigen receptors targeted to PSCA
    Article Snippet: Cell Lines: (Human metastatic prostate cancer cell lines DU145 (ATCC HTB-81) and PC-3 (ATCC CRL-1435) were cultured in RPMI-1640 (Lonza) containing 10% fetal bovine serum (FBS, Hyclone), and 1× antibiotic-antimycotic (Gibco) containing 100 U/mL penicillin, 100 μg/mL streptomycin, and 0.25 ug/mL fungizone (complete RPMI). .. The human fibrosarcoma cell line, HT1080 (ATCC CCL-121), and the human embryonic kidney cell line, 293T (ATCC CRL-3216), were cultured in Dulbecco's Modified Eagles Medium (DMEM, Life Technologies) containing 10% FBS, 1× antibiotic-antimycotic, 25 mM HEPES (Irvine Scientific), and 2 mM L-Glutamine (Fisher Scientific) (complete DMEM). .. The human prostate cancer xenograft LAPC-9 (a kind gift from Dr. Robert Reiter, UCLA) was cultured in Iscove's Modified Dulbecco's Medium (IMDM, Irvine Scientific) containing 20% FBS and 1× antibiotic-antimycotic (complete IMDM).



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    ATCC ht1080 human fibrosarcoma cell line
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    ATCC human fibrosarcoma cell line ht1080 127
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    human fibrosarcoma cell line ht1080 127 - by Bioz Stars, 2026-09
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